Journal: Scientific Reports

Article Title: Ginkgolic acid inhibits fusion of enveloped viruses

doi: 10.1038/s41598-020-61700-0

Figure Lengend Snippet: GA inhibits ZIKV infection. ( A ) Viability of NHA infected with ZIKV and treated with GA. NHA were grown in 24-well plates to >80% confluency and treated with GA (0–20 µM) or DMSO for 3 hours and infected with ZIKV strain PRVABC59 at an MOI of 0.3. At 12 hpi, supernatants were carefully removed and replaced with fresh AGM. ( B ) 7 days post infection, samples were analyzed for cell viability by the MTS assay and then live cells were harvested for RNA and processed with Taqman based real-time PCR to quantify ZIKV RNA. Experiments were performed in duplicate three independent times and the data were analyzed by t-test (* indicates p ≤ 0.05). ( C ) Cell free ZIKV in 199V medium was pretreated with 10 µM GA or with DMSO for 1 hour at 37 °C. The pretreated ZIKV was used to infect Vero cells grown in 6 well plates >80% confluency with 0.5 MOI for 1 hour. The infected cells were washed twice with warm DPBS and supplemented with DMEM supplemented with 5% FBS. Supernatant was collected at 4, 24 and 48 hours post-inoculation for cell free viral RNA copy number determination. Experiments were performed in triplicates.

Article Snippet: ZIKV strain PRVABC59 obtained from the American Type Culture Collection (ATCC; Manassas, VA) was propagated in Vero cells grown in T-150 flasks by infection at 1:50 dilution of viral stock (MOI 0.25) in the absence of FBS.

Techniques: Infection, MTS Assay, Real-time Polymerase Chain Reaction

Journal: Nutrients

Article Title: Novel Therapeutic Nutrients Molecules That Protect against Zika Virus Infection with a Special Note on Palmitoleate

doi: 10.3390/nu15010124

Figure Lengend Snippet: List of novel nutrient molecules and mechanism of protection against ZIKV infection.

Article Snippet: Palmatine , ATCC VR-1843 , Vero cells , Prevents ZIKV binding and entry , Virucidal effects , [ ] .

Techniques: Infection, Activity Assay, Derivative Assay, Binding Assay, Inhibition, Glycoproteomics, Virus, Isolation, In Vitro, In Vivo, Blocking Assay, Membrane, Recombinant, Expressing, Concentration Assay